Part:BBa_K2302010:Design

T7-His-PelB+CALB+T7terminater

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1079
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 81
  Illegal NgoMIV site found at 107
  Illegal NgoMIV site found at 800
  Illegal NgoMIV site found at 910
  Illegal AgeI site found at 602
  Illegal AgeI site found at 860
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

for optimal expression, the plasmid should be transformed into E. coli (DE3).

Source

pelB and CALB are synthetic cDNAs. They have been subcloned into the expression plasmid pET-14b.